MLT- Professional 26 Jan Welcome to your MLT- Professional Name: Email: Phone Number: [A quantitative sandwich enzyme immunoassay for intact serum hCG was performed on week 4 and the result was 40,000 mIU/mL (reference range 10,000–80,000 mIU/mL). The physician suspected a molar pregnancy and requested that the laboratory repeat the test checking for the hook effect. Which process would identify this problem?] Obtain a new plasma specimen and heat inactivate before testing Obtain a urine specimen and perform the assay Perform a qualitative pregnancy test Perform a serial dilution of the sample and repeat the test None . [Which assay is used to confirm difficult cases of hypothyroidism?] Free T3 assay Free thyroxine index Thyrotropin-releasing hormone (TRH) stimulation test TBG assay None . [The following results were obtained from a pure culture of gram-negative rods recovered from the pulmonary secretions of a 10-year-old cystic fibrosis patient with pneumonia: Oxidase = + Motility = + Glucose OF (open) = + Gelatin hydrolysis = + Pigment = Red Arginine dihydrolase = + (nonfluorescent) Growth at 42°C = + Flagella = + (polar, monotrichous) Which is the most likely organism?] Burkholderia pseudomallei Pseudomonas stutzeri Burkholderia cepacia Pseudomonas aeruginosa None . [A person has a fasting triglyceride level of 240 mg/dL. The physician wishes to know the patient’s non-HDL cholesterol level. What cholesterol fractions should be measured?] Total cholesterol and HDL cholesterol Total cholesterol and LDL cholesterol HDL cholesterol and LDL cholesterol Total cholesterol and chylomicrons None . [Which of the following assays is used to determine the risk of developing cancer?] Epidermal growth factor receptor (EGF-R) Squamous cell carcinoma antigen (SCC) c-erb B-2 gene expression p53 gene mutation None . [Which electrolyte measurement is least affected by hemolysis?] Potassium Calcium Pi Magnesium None . [Which of the following would cause you to suspect that a thrombus had formed in the artery while you were collecting an ABG?] A hematoma quickly forms at the site. The patient complains of extreme pain. The pulse distal to the site is very weak. All of the above. None . [Patient and Family HistoryA 45-year-old woman visited her doctor complaining of easy bruising and menorrhagia occurring for the past few weeks. The patient had no history of excessive bleeding during childbirth several years earlier nor during a tonsillectomy in childhood. Her family history was unremarkable. These clinical manifestations and laboratory results are consistent with:] Factor VIII inhibitor Factor V inhibitor Factor VIII deficiency Lupus anticoagulant None Comment . [The ortho-nitrophenyl-β-galactopyranoside (ONPG) test is most useful when differentiating:] Salmonella spp. from Pseudomonas spp. Shigella spp. from some strains of Escherichia coli Klebsiella spp. from Enterobacter spp. Proteus vulgaris from Salmonella spp. None . [A urine sample has a negative blood reaction and 5–10 cells per high-power field that resemble red blood cells. What is the best course of action?] Mix a drop of sediment with 1 drop of WBC counting fluid and reexamine Report the results without further testing Repeat the blood test and if negative report the results If the leukocyte esterase test is positive, report the cells as WBCs None . [In which of the following conditions does LAP show the least activity?] Leukemoid reactions Idiopathic myelofibrosis PV CML None . [Creatinine is formed from the:] Oxidation of creatine Oxidation of protein Deamination of dibasic amino acids Metabolism of purines None . [Given the following dry reagent strip urinalysis results, select the most appropriate course of action: pH = 8.0 Protein = 1+ Glucose = Neg Blood = Neg Ketone = Neg Nitrite = Neg Bilirubin = Neg] Report the results, assuming acceptable quality control Check pH with a pH meter before reporting Perform a turbidimetric protein test and report instead of the dipstick protein Request a new specimen None . [A 45-year-old hunter developed fever, myalgia, and periorbital edema. He has a history of bear meat consumption. The most likely causative agent is:] Toxoplasma gondii Taenia solium Hymenolepis nana Trichinella spiralis None . [Which of the following CSF test results is most commonly increased in patients with multiple sclerosis?] Glutamine Lactate IgG index Ammonia None . [Which of the following conditions is associated with an increase in ionized calcium (Cai) in the blood?] Alkalosis Hypoparathyroidism Hyperalbuminemia Malignancy None . [Plate 50 is a Wright’s-stained peripheral blood film, 1,000×. Which description best characterizes the morphology of the neutrophil shown in this plate?] Normal morphology Döhle bodies Toxic granulation Hypersegmentation None Comment . [Cetrimide agar is used as a selective isolation agar for which organism?] Acinetobacter spp. Pseudomonas aeruginosa Moraxella spp. Stenotrophomonas maltophilia None . [Antibodies to thyroid peroxidase can be detected by using agglutination assays. Which of the following diseases may show positive results with this type of assay?] Graves’ disease and Hashimoto’s thyroiditis Myasthenia gravis Granulomatous thyroid disease Addison’s disease None . [Which tumor marker is used to determine the usefulness of trastuzumab (Herceptin) therapy for breast cancer?] PR CEA HER-2/neu Myc None . [Which of the following effects results from exposure of a normal arterial blood sample to room air?] PO2 increased PCO2 decreased pH increased PO2 decreased PCO2 increased pH decreased PO2 increased PCO2 decreased pH decreased PO2 decreased PCO2 decreased pH decreased None . [A solvent needed for HPLC requires a 20.0 mmol/L phosphoric acid buffer, pH 3.50, made by mixing KH2PO4 and H3PO4. How many grams of KH2PO4 are required to make 1.0 L of this buffer? Formula weights: KH2PO4 = 136.1; H3PO4 = 98.0; pKa H3PO4 = 2.12] 1.96 g 2.61 g 2.72 g 19.2 g None . [Which inclusions may be seen in leukocytes?] Dohle bodies Basophilic stippling Malarial parasites Howell–Jolly bodies None . [Which of the following is the best, rapid, noncultural test to perform when Gardnerella vaginalis is suspected in a patient with vaginosis?] 10% KOH test 3% H2O2 test 30% H2O2 test All of these options None . [Which of the following statements is true?] Apoenzyme + prosthetic group = holoenzyme A coenzyme is an inorganic molecule required for activity Cofactors are as tightly bound to the enzyme as prosthetic groups All enzymes have optimal activity at pH 7.00 None . [A manual white blood cell (WBC) count was performed. A total of 36 cells were counted in all 9-mm2 squares of a Neubauer-ruled hemacytometer. A 1:10 dilution was used. What is the WBC count?] 0.4 × 109/L 2.5 × 109/L 4.0 × 109/L 8.0 × 109/L None . [The single most important means of preventing the spread of infection is:] proper hand antisepsis. keeping clothes clean. wearing a mask. wearing gloves. None . [The following results were obtained from a pure culture of gram-negative rods recovered from the pulmonary secretions of a 10-year-old cystic fibrosis patient with pneumonia: Oxidase = + Motility = + Glucose OF Gelatin hydrolysis = + (open) = + Pigment = Red Arginine dihydrolase = + (nonfluorescent) Growth at 42°C = + Flagella = + (polar monotrichous) Which is the most likely organism?] Burkholderia pseudomallei Pseudomonas stutzeri Burkholderia cepacia Pseudomonas aeruginosa None . [According to American Diabetes Association criteria, which result is consistent with a diagnosis of impaired fasting glucose?] 99 mg/dL 117 mg/dL 126 mg/dL 135 mg/dL None . [The test used most often to separate the Micrococcaceae family from the Streptococcaceae family is:] Bacitracin Catalase Hemolysis pattern All of these options None . Time's up