New Quiz 10 Dec Welcome to your MLT- Professional Name: Email: Phone Number: [The following conditions are all causes of alkalosis. Which condition is associated with respiratory (rather than metabolic) alkalosis?] Anxiety Hypovolemia Hyperaldosteronism Hypoparathyroidism None . [Plate 11 is a photomicrograph of a fungal slide culture stained with lactophenol cotton blue, 400×. The morphology is most consistent with which fungus?] Aspergillus spp. Penicillium spp. Scedosporium Fusarium None Comment . [What is the maximum recommended storage time and temperature for an arterial blood gas sample drawn in a plastic syringe?Storage Time - Temperature] 10 min - 2°C–8°C 20 min - 2°C–8°C 30 min - 2°C–8°C 30 min - 22°C None Comment . [The following figure shows a DNA size standard (ladder) made by restriction enzyme digestion (PstI) of lambda phage DNA that has been separated by agarose gel electrophoresis. Which DNA band has the highest molecular weight? (fig 25)] 1 2 3 4 None Comment . [In testing for DiGeorge’s syndrome, what type of laboratory analysis would be most helpful in determining the number of mature T cells?] Complete blood count Nitroblue tetrazolium (NBT) test T-cell enzyme assays Flow cytometry None . [The most rapid definitive diagnosis of a genital herpes simplex (HSV-2) infection in a 20-year-old man is made by which method?] Direct immunofluorescence test for viral antigen in vesicle fluid Titer of serum and seminal fluid for antibodies to herpes simplex Detection of antiherpes simplex in seminal fluid Cell culture of vesicle fluid None . [Alcaligenes faecalis (formerly A. odorans) is distinguished from Bordetella bronchiseptica with which test?] Urease (rapid) Oxidase Growth on MacConkey agar Motility None . [In which condition would hypochloremia be expected?] Respiratory alkalosis Metabolic acidosis Metabolic alkalosis All of these options None . [Which group of streptococci is associated with erythrogenic toxin production?] Group A Group B Group C Group G None . [Which of the following is an appropriate screening test for the diagnosis of lupus anticoagulant?] Thrombin time test Diluted Russell’s viper venom test (DRVVT) D-dimer test FDP test None . [Which of the following is the most effective serial sampling time for ruling out AMI using both myoglobin and a cardiac specific marker in an emergency department environment?] Admission and every hour for the next 3 hours or until positive Admission, 2 hours, 4 hours, and 6 hours or until positive Admission, 3 hours, 6 hours, and a final sample within 12 hours Admission and one sample every 8 hours for 48 hours None . [What is the disposition of a donor red blood cell unit that contains an antibody?] The unit must be discarded Only the plasma may be used to make components The antibody must be adsorbed from the unit The unit may be labeled indicating it contains antibody and released into inventory None . [A culture from an intra-abdominal abscess produced orange-tan colonies on blood agar that gave the following results: Oxidase = + Nitrate reduction = + KIA = Alk/Alk (H2S)+ Motility = + (single polar flagellum) DNase = + Ornithine decarboxylase = + Growth at 42°C = Neg The most likely identification is:] Shewanella putrefaciens Acinetobacter spp. Pseudomonas aeruginosa Chryseobacterium spp. None . [Which of the following liver diseases produces the highest levels of transaminases?] Hepatic cirrhosis Obstructive jaundice Hepatic cancer Alcoholic hepatitis None . [While on a 7-day cruise to Vancouver and Alaska, a number of passengers reported to the ship’s medical staff complaining of vomiting and diarrhea. Which is the most likely virus to have infected these mainly adult passengers?] Rotavirus Parainfluenza Respiratory syncytial Norwalk or Norwalk-like viruses None . [Which of the following methods used for HIV identification is considered a signal amplification technique?] Branched-chain DNA analysis DNA PCR Reverse transcriptase PCR Nucleic acid sequence–based assay (NASBA) None . [Which statement accurately describes the process of fluorescent in situ hybridization (FISH)?] Hybridization is performed on DNA extracted from cells Hybridization is performed directly on intact chromosomes Hybridization probes are attached to histones associated with the chromosomes Hybridization occurs by attachment to the probe only at the centromere None . [Select the correct order of Hgb migration on agarose or cellulose acetate at pH 8.6.] – C→F→S→A + – S→C→A→F + – C→S→F→A + – S→F→A→C + None . [The study of pharmacogenomics involves which type of testing?] Family studies to determine the inheritance of drug resistance Testing drugs with cell cultures to determine the minimum toxic dosage Testing for single nucleotide polymorphisms known to affect drug metabolism Comparison of dose-response curves between family members None . [Which of the following is the most critical part of blood culture collection?] Adequately filling two media vials Antisepsis of the collection site Selecting the collection site Timing of the second set of cultures None . [In addition to CO2 requirements and biochemical characteristics, Brucella melitensis and Brucella abortus are differentiated by growth on media containing which two dyes?] Basic fuchsin and thionin Methylene blue and crystal violet Carbol fuchsin and iodine Safranin and methylene blue None . [Which of the following mechanisms is responsible for metabolic acidosis?] Bicarbonate deficiency Excessive retention of dissolved CO2 Accumulation of volatile acids Hyperaldosteronism None . [Which of the following dyes are used in Sternheimer–Malbin stain?] Hematoxylin and eosin Crystal violet and safranin Methylene blue and eosin Methylene blue and safranin None . [In addition to the number of true negatives (TN), which of the following measurements is needed to calculate specificity?] True positives Prevalence False negatives False positives None . [Blood is crossmatched on an A positive person with a negative antibody screen. The patient received a transfusion of A positive RBCs 3 years ago. The donors chosen for crossmatch were A positive. The crossmatch was run on the Ortho Provue and yielded 3+ incompatibility. How can these results be explained?] The patient has an antibody to a low-frequency antigen The patient has an antibody to a high-frequency antigen The patient is an A2 with anti-A1 The patient is an A1 with anti-A2 None . [Which PCO2 value would be seen in maximally compensated metabolic acidosis?] 15 mm Hg 30 mm Hg 40 mm Hg 60 mm Hg None . [In flow cytometry, the term “gating” refers to:] Selection of a subpopulation of cells to count Determining the fluorescent emission spectrum of cells of interest Interference caused by binding of more than a single antibody Selecting the appropriate counting aperture None . [Which of the following compounds can interfere with the coulometric chloride assay?] Bromide Ascorbate Acetoacetate Nitrate None . [An anti-M reacts strongly through all phases of testing. Which of the following techniques would not contribute to removing this reactivity so that more clinically significant antibodies may be revealed?] Acidifying the serum Prewarmed technique Adsorption with homozygous cells Testing with enzyme-treated red cells None . [The four essential functions of a manager are:] Staffing, decision making, cost analysis, evaluating Directing, leading, forecasting, implementing Planning, organizing, directing, controlling Innovating, designing, coordinating, problem solving None . Time's up
