Welcome to your MLT- Professional Name: Email: Phone Number: [Which of the following methods for HDL cholesterol is the reference method?] Manganese–heparin Magnesium–phosphotungstate Magnesium–dextran Ultracentrifugation None . [What is the main use of laboratory tests to detect antibodies to islet cells and insulin in cases of insulin-dependent diabetes mellitus (IDDM)?] To regulate levels of injected insulin To diagnose IDDM To rule out the presence of other autoimmune diseases To screen susceptible individuals prior to destruction of β cells None . [Which of the following procedures can be used to detect proportional error in a new method for glucose?] Compare the standard deviation of 40 patient samples to the hexokinase method Measure a mixture made from equal parts of normal and high-QC sera Add 5.0 mg of glucose to 1.0 mL of a serum of known concentration and measure Compare the mean of 40 normal samples to the hexokinase method None . [A sample of amniotic fluid collected for fetal lung maturity studies from a woman with a pregnancy compromised by hemolytic disease of the newborn (HDN) has a creatinine of 88 mg/dL. What is the most likely cause of this result?] The specimen is contaminated with blood Bilirubin has interfered with the measurement of creatinine A random error occurred when the absorbance signal was being processed by the analyzer The fluid is urine from accidental puncture of the urinary bladder None . [Mean cell volume (MCV) is calculated using the following formula:] (Hgb ÷ RBC) × 10 (Hct ÷ RBC) × 10 (Hct ÷ Hgb) × 100 (Hgb ÷ RBC) × 100 None . [What is the clinical significance of K-ras testing?] K-Ras mutations make tumor cells more susceptible to chemotherapy K-Ras is a tumor suppressor gene and mutations are associated with increased lifetime risk of malignancy K-Ras mutations result in treatment resistance to growth factor receptor inhibitors K-Ras is used to identify the tissue of origin None . [SITUATION: A lipemic specimen collected from an adult after a 12-hour fast was assayed for total cholesterol, triglycerides, and HDL cholesterol using a direct HDL method. Following are the results: Total cholesterol = 220 mg/dL HDL cholesterol = 40 mg/dL Triglyceride = 420 mg/dL The physician requests an LDL cholesterol assay after receiving the results. How should the LDL cholesterol be determined?] Dilute the specimen 1:10 and repeat all tests; calculate LDL cholesterol using the Friedewald equation Perform a direct LDL cholesterol assay Ultracentrifuge the sample and repeat the HDL cholesterol on the infranate. Use the new result to calculate the LDL cholesterol Repeat the HDL cholesterol using the manganese heparin precipitation method. Use the new result to calculate the LDL cholesterol None . [Required PPE when processing specimens excludes:] chin-length face shields. disposable gloves. fluid-resistant lab coats. fluid-resistant shoe covers. None . [According to federal and state regulations, a hospital’s capital budget should include which of the following before projects that cost $150,000 can be submitted for approval?] A time table of completion A cost analysis Salaries and wages for new employees A certificate of need None . [Select the most sensitive recovery method for Acanthamoeba spp. from lens care solutions or corneal biopsies.] The trichrome staining method The use of monoclonal reagents for the detection of antibody The use of non-nutrient agar cultures seeded with Escherichia coli The Giemsa’s stain method None . [A new employee’s performance is to be evaluated at the end of his or her probationary period, and must relate to:] The person’s job description Verbal instructions given Wage and salary policies Recruitment practices None . [Upon which principle is the biuret method based?] The reaction of phenolic groups with CuIISO4 Coordinate bonds between Cu+2 and carbonyl and imine groups of peptide bonds. The protein error of indicator effect producing color when dyes bind protein The reaction of phosphomolybdic acid with protein None . [Which biochemical tests should be performed in order to identify colorless colonies growing on MacConkey agar (swarming colonies on blood agar) from a catheterized urine specimen?] Indole, phenylalanine deaminase, and urease Glucose, oxidase, and lactose utilization Phenylalanine deaminase and bile solubility H2S and catalase None . [Which genotype(s) will give rise to the Bombay phenotype?] HH only HH and Hh Hh and hh hh only None . [Which of the following specimens should be protected from light?] BUN CBC Bilirubin Glucose None . [In the event of a body fluid splash to the eyes, the victim should immediately:] call 1122 so the paramedics will come. flush eyes with water for 10 minutes. go to the emergency room. wipe the eyes with a tissue. None . [Inflammation involves a variety of biochemical and cellular mediators. Which of the following may be increased within 72 hours after an initial infection?] Neutrophils, macrophages, antibody, complement, α1-antitrypsin Macrophages, T cells, antibody, haptoglobin, fibrinogen Neutrophils, macrophages, complement, fibrinogen, C-reactive protein Macrophages, T cells, B cells, ceruloplasmin, complement None . [Which of the following conditions is cause for rejecting an analytical run?] Two consecutive controls greater than 2 s above or below the mean Three consecutive controls greater than 1 s above the mean Four controls steadily increasing in value but less than ±1 s from the mean One control above +1 s and the other below -1 s from the mean None . [A blood-tainted pleural fluid is submitted for culture. Which test result would be most conclusive in classifying the fluid as an exudate?] LD fluid/serum = 0.65 Total protein = 3.2 g/dL RBC count = 10,000/μL WBC count = 1,500/μL None . [In the Kauffmann–White schema, the combined antigens used for serological identification of the Salmonella spp. are:] O antigens H antigens Vi and H antigens O, Vi, and H antigens None . [A patient receives a transfusion of packed red cells and fresh frozen plasma and develops an anaphylactic, nonhemolytic reaction. She reports receiving a transfusion 20 years earlier. She had no reaction to the previous transfusion, but she did feel “poorly” a few weeks later. Which of the following transfused substances most likely elicited the reaction?] IgA Group A antigen Rho (D) antigen An antigen belonging to the Duffy system None . [Which analyte should be reported as a ratio using creatinine concentration as a reference?] Urinary microalbumin Urinary estriol Urinary sodium Urinary urea None . [Plate 17 is an unstained fecal specimen seen under 400× using brightfield microscopy. The plate shows the ovum of which parasite?] Fasciola hepatica Paragonimus westermani Metagonimus yokogawai Opisthorchis viverrini None Comment . [A CSF CytoPrep smear shows many smudge cells and macrophages with torn cell membranes. What most likely caused this problem?] Failure to add albumin to the cytospin cup Failure to collect the CSF in EDTA Centrifuge speed too low Improper alignment None . [An HPLC assay for procainamide gives an internal standard peak that is 15% greater in area and height for sample 1 than sample 2. The technologist should suspect that:] The column pressure increased while sample 2 was being analyzed Less recovery from sample 2 occurred in the extraction step The pH of the mobile phase increased during chromatography of sample 2 There was more procainamide in sample 1 thansample 2 None . [Quantitation of a drug by gas chromatography–mass spectroscopy (GC-MS) is usually performed in which mode?] Total ion chromatography Selective ion monitoring Ion subtraction Selective reaction monitoring None . [Which of the following conditions is least likely to be detected by dry reagent strip tests for proteinuria?] Orthostatic albuminuria Chronic renal failure Pyelonephritis Renal tubular proteinuria None . [Nutritionally variant streptococci (NVS) require specific thiol compounds, cysteine, or the active form of vitamin B6. Which of the following tests supplies these requirements?] CAMP test Bacitracin susceptibility test Bile solubility test Staphylococcal cross-streak test None . [Which of the following is a characteristic of low molecular weight heparin (LMWH)?] Generally requires monitoring Specifically acts on factor Va Has a longer half-life than unfractionated heparin Can be used as a fibrinolytic agent None . [Which group of streptococci is associated with erythrogenic toxin production?] Group A Group B Group C Group G None . Time's up