Welcome to your MLT- Professional Name: Email: Phone Number: [Four blood cultures were taken over a 24-hour period from a 20-year-old woman with severe diarrhea. The cultures grew motile (room temperature), gram-negative rods. A urine specimen obtained by catheterization also showed gram-negative rods, 100,000 col/mL. Given the following results, which is the most likely organism? TSI = A/A gas Indole = + VP = Neg MR = + H2S = Neg Citrate = Neg Urease = Neg Lysine decarboxylase = + Phenylalanine deaminase = Neg] Proteus vulgaris Salmonella typhi Yersinia enterocolitica E. coli None . [According to the College of American Pathologists (CAP) guidelines, which services for mycobacteria would be performed by a Level II laboratory?] No procedures performed Acid-fast staining, inoculation, and referral to a reference laboratory Isolation and identification of Mycobacterium tuberculosis; preliminary identification of other species Definitive identification of all mycobacteria None . [Which of the following conditions is most often associated with normal CSF glucose and protein?] Multiple sclerosis Malignancy Subarachnoid hemorrhage Viral meningitis None . [Criterion-referenced examinations are used in order to determine the:] Competency of a student according to a predetermined standard Validity of a test Status of one student compared to the whole group Accuracy of a test None . [Urine production of less than 400 mL/day is:] Consistent with normal renal function and H2O balance Termed isosthenuria Defined as oliguria Associated with diabetes mellitus None . [What influence does the Philadelphia (Ph1) chromosome have on the prognosis of patients with chronic myelocytic leukemia?] It is not predictive The prognosis is better if Ph1 is present The prognosis is worse if Ph1 is present The disease usually transforms into AML when Ph1 is present None . [Iron deficiency anemia may be distinguished from anemia of chronic infection by:] Serum iron level Red cell morphology Red cell indices Total iron-binding capacity None . [What is a general definition for autoimmunity?] Increase of tolerance to self-antigens Loss of tolerance to self-antigens Increase in clonal deletion of mutant cells Manifestation of immunosuppression None . [The preferred vein for venipuncture in the “H” pattern is the:] accessory cephalic. basilic. cephalic. median cubital. None . [Serial TnI assays are ordered on a patient at admission, 3 hours, and 6 hours afterwards. The samples were collected in heparinized plasma separator tubes. Following are the results (reference range 0–0.03 μg/L) Admission = 3 hours = 6 hours = 0.03 μg/L 0.07 μg/L 0.02 μg/L These results indicate:] A positive test for acute myocardial infarction Unstable angina Cardiac injury of severity less than myocardial infarction Random error with the 3-hour sample None . [An ANA test on HEp-2 cells shows nucleolar staining in interphase cells and dense chromatin staining in mitotic cells. The most likely cause of this staining pattern is:] Antifibrillarin antibody Antiribosomal p antibody A serum with nucleolar and homogeneous patterns Technical artifact None . [Plate 44 is a Wright’s-stained peripheral blood film, 1,000×. The white blood cells in this field are negative for peroxidase, chloroacetate esterase, and Sudan B. The cells are positive for terminal deoxynucleotidyl transferase (Tdt) and PAS. On the basis of these findings, what is the most appropriate classification of these cells?] Lymphoblasts with L1 morphology Lymphoblasts with L2 morphology Lymphoblasts with L3 morphology Chronic lymphocytic leukemia cells None Comment . [Factor V Leiden promotes thrombosis by preventing:] Deactivation of factor Va Activation of factor V Activation of protein C Activation of protein S None . [Which region of the immunoglobulin molecule can bind antigen?] Fab Fc CL CH None . [Culture of a skin (hand) wound from a manager of a tropical fish store grew on Lowenstein–Jensen agar slants at 30°C in 10 days but did not grow on the same media at 37°C in 20 days. Given the following results, what is the most likely identification? Photochromogen = + Niacin = Neg Urease = + Heat-stable catalase (68°C) = Neg Nitrate reduction = Neg Tween 80 hydrolysis = +] Mycobacterium marinum Mycobacterium kansasii Mycobacterium avium-intracellulare complex Mycobacterium tuberculosis None . [Which support medium can be used to determine the molecular weight of a protein?] Cellulose acetate Polyacrylamide gel Agar gel Agarose gel None . [Can crossmatching be performed on October 14th using a patient sample drawn on October 12th?] Yes, a new sample would not be needed Yes, but only if the previous sample has no alloantibodies No, a new sample is needed because the 2-day limit has expired No, a new sample is needed for each testing None . [Select the amino acid substitution that is responsible for sickle cell anemia.] Lysine is substituted for glutamic acid at the sixth position of the α-chain Valine is substituted for glutamic acid at the sixth position of the β-chain Valine is substituted for glutamic acid at the sixth position of the α-chain Glutamine is substituted for glutamic acid at the sixth position of the β-chain None . [Which of the following characteristics is higher for synovial fluid than for the serous fluids?] SG Glucose Total protein Viscosity None . [Variation in red cell size observed on the peripheral smear is described as:] Anisocytosis Hypochromia Poikilocytosis Pleocytosis None . [The thick muscle layer of the heart is called the:] endocardium. epicardium. myocardium. pericardium. None . [An 80-year-old male patient was admitted to the hospital with a fever of 102°F. A sputum culture revealed many gram-negative rods on MacConkey agar and blood agar. The patient was diagnosed with pneumonia. The following biochemical results were obtained from the culture: H2S = Neg Lactose = + Urease = + Citrate = + Indole = + VP = + Motility = Neg Resistance to ampicillin and carbenicillin What is the most likely identification?] Klebsiella oxytoca Proteus mirabilis Escherichia coli Klebsiella pneumoniae None . [A donor was found to contain anti-K using pilot tubes from the collection procedure. How would this affect the compatibility test?] The AHG major crossmatch would be positive The IS (immediate spin) major crossmatch would be positive The recipient’s antibody screen would be positive for anti-K Compatibility testing would not be affected None . [Which laboratory test is used to screen for activated protein C resistance?] Mixing studies with normal plasma Mixing studies with factor-deficient plasma Modified APTT with and without activated protein C Modified PT with and without activated protein C None . [Which atmospheric condition is needed to recover Campylobacter spp. from specimens inoculated onto a Campy-selective agar at 35°C–37°C and 42°C?] 5% O2, 10% CO2, and 85% N2 20% O2, 10% CO2, and 70% N2 20% O2, 20% CO2, and 60% N2 20% O2, 5% CO2, and 75% N2 None . [What substance may be measured as an alternative to creatinine for evaluating GFR?] Plasma urea Cystatin C Uric acid Potassium None . [In thin-layer chromatography (TLC), the distance the solute migrates divided by the distance the solvent migrates is the:] tR Kd Rf pK None . [Which of the following tests must have a 9:1 ratio of blood to anticoagulant in the collection tube?] 2-hour PP Blood culture Electrolytes Protime None . [The k (Cellano) antigen is a high-frequency antigen and is found on most red cells. How often would one expect to find the corresponding antibody?] Often, because it is a high frequency antibody Rarely, because most individuals have the antigen and therefore would not develop the antibody It depends upon the population, because certain racial and ethnic groups show a higher frequency of anti-k Impossible to determine without consulting regional blood group antigen charts None . [Which of the following organisms accounts for the majority of septic arthritis cases in young and middle-age adults?] H. influenzae Neisseria gonorrhoeae Staphylococcus aureus Borrelia burgdorferi None . Time's up
