Welcome to your MLT- Professional Name: Email: Phone Number: [The spinal cord and brain are covered by protective membranes called:] meninges. neurons. papillae. viscera. None . [Given a situation where screening cells, major crossmatch, autocontrol, and DAT (anti-IgG) are all positive, what procedure should be performed next?] Adsorption using rabbit stroma Antigen typing of patient cells Elution followed by a cell panel on the eluate Selected cell panel None . [What is the most likely cause when a Western blot or ELISA is positive for all controls and samples?] Improper pipetting Improper washing Improper addition of sample Improper reading None . [Refer to the following scatterplot, histograms, and automated values on a 45-year-old man. What follow-up verification procedure is indicated before releasing these results?(fig)] Redraw blood sample using a sodium citrate tube; multiply PLTs × 1.11 Dilute the WBCs 1:10; multiply × 10 Perform plasma blank Hgb to correct for lipemia Warm specimen at 37°C for 15 minutes; rerun specimen None Comment . [In real-time PCR, what value is needed in order to determine the threshold?] Background signal Melt temperature Maximum fluorescence Threshold cycle None . [What is the main advantage of the recovery and reinfusion of autologous stem cells?] It slows the rate of rejection of transplanted cells It prevents graft-versus-host disease No HLA testing is required Engraftment occurs in a more efficient sequence None . [Which order of events should be followed at the conclusion of a laboratory worker’s shift in order to prevent the spread of bloodborne pathogens?] Remove gloves, disinfect area, wash hands, remove lab coat Disinfect area, remove gloves, remove lab coat, wash hands Disinfect area, remove gloves, wash hands, remove lab coat Remove gloves, wash hands, remove lab coat, disinfect area None . [SITUATION: A 5-mL urine specimen is submitted for routine urinalysis and analyzed immediately. The SG of the sample is 1.012 and the pH is 6.5. The dry reagent strip test for blood is a large positive (3+) and the microscopic examination shows 11–20 RBCs per HPF. The leukocyte esterase reaction is a small positive (1+), and the microscopic examination shows 0–5 WBCs per HPF. What is the most likely cause of these results?] Myoglobin is present in the sample Free hemoglobin is present Insufficient volume is causing microscopic results to be underestimated Some WBCs have been misidentified as RBCs None . [Micrococcus and Staphylococcus species are differentiated by which test(s)?] Fermentation of glucose (OF tube) Catalase test Gram stain All of these options None . [An IFE revealed excessive amounts of polyclonal IgM and low concentrations of IgG and IgA. What is the most likely explanation of these findings and the best course of action?] Proper amounts of antisera were not added; repeat both tests Test specimen was not added properly; repeat both procedures Patient has common variable immunodeficiency; perform B-cell count Patient has immunodeficiency with hyper-M; perform immunoglobulin levels None . [A striking feature of the peripheral blood of a patient with CML is a:] Profusion of bizarre blast cells Normal number of typical granulocytes Presence of granulocytes at different stages of development Pancytopenia None . [Which statement is correct regarding sample storage for the prothrombin time test?] Stable for 24 hours if the sample is capped Stable for 24 hours if the sample is refrigerated at 4°C Stable for 4 hours if the sample is stored at 4°C Should be run within 8 hours None . [TDM peak concentration may be defined as the:] highest concentration of the drug during a dosing interval. lowest concentration of the drug during a dosing interval. concentation required for maximum drug effectiveness. none of the above. None . [Which type of monochromator produces the purest monochromatic light in the UV range?] A diffraction grating and a fixed exit slit A sharp cutoff filter and a variable exit slit Interference filters and a variable exit slit A prism and a variable exit slit None . [Which nonblood specimen is most frequently analyzed in the lab?] CSF Pleural fluid Synovial fluid Urine None . [A 61-year-old male with a history of multiple myeloma had a stem cell transplant 3 years ago. The donor was O positive and the recipient was B positive. He is admitted to a community hospital for fatigue and nausea. Typing results reveal the following: Anti-A = 0 Anti-B =0 Anti-A,B = 0 Anti-D = 4+ A1 cells = 4+ B cells = 0 How would you report this type?] O positive B positive A positive Undetermined None . [Blood sample collection time for peak drug levels:] Varies with the drug, depending on its rate of absorption Is independent of drug formulation Is independent of the route of administration Is 30 minutes after a bolus intravenous injection is completed None . [SITUATION: A 53-year-old woman donates blood at her place of employment. She weighs 150 lb and has a hemoglobin of 13 g/dL. She is currently on warfarin and vitamin B12. Is she an acceptable donor?] Yes No, she is on warfarin Yes, for red cells only No, her hemoglobin is too low None . 0.4 7.2 10.6 11.0 None Comment . [Several hours after birth, a baby boy develops petechiae and purpura and a hemorrhagic diathesis. The platelet count is 18 × 10^9/L. What is the most likely explanation for the low platelet count?] Drug-induced thrombocytopenia Secondary thrombocytopenia Neonatal alloimmune thrombocytopenia Neonatal DIC None Comment . [Which statement best describes immunofixation electrophoresis (IEF)?] Proteins are separated by electrophoresis followed by overlay of monospecific anti-immunoglobulins Proteins react with monospecific antisera followed by electrophoresis Antisera are electrophoresed, then diffused against patient’s serum Serum is electrophoresed; the separated immunoglobulins diffuse against specific antisera placed into troughs None . [You are evaluating an ELISA assay as a replacement for your immunofluorescent antinuclear antibody test. You test 50 specimens in duplicate on each assay. The ELISA assay uses a HEp-2 extract as its antigen source. The correlation between the ELISA and the IFA tests is only 60% (30 of 50 specimens agree). Which of the following is the next best course of action?] Test another 50 specimens Perform a competency check on the technologists who performed the tests Order a new lot of both kits and then retest on the new lots Refer the discrepant specimens for testing by another method None . [Which of the following plots is best for comparison of precision and accuracy amonglaboratories?] Levy–Jennings Tonks–Youden Cusum Linear regression None Comment . [Which of the following bands would constitute a positive Western Blot for HIV?] p24, gp41, p17 p55, gp120, p51 gp160, p31, p56 p24, p30, p55 None . [Which of the following tests best differentiates Shigella species from E. coli?] Hydrogen sulfide, VP, citrate, and urease Lactose, indole, ONPG, and motility Hydrogen sulfide, MR, citrate, and urease Gas, citrate, and VP None . [The most suitable product for treatment of factor VIII deficiency is:] Fresh frozen plasma Factor VIII concentrate Prothrombin complex concentrate Factor V Leiden None . [The thick muscle layer of the heart is called the:] endocardium. epicardium. myocardium. pericardium. None . [What is the interpretation when an Ouchterlony plate shows crossed lines between wells 1 and 2 (antigen is placed in the center well and antisera in wells 1 and 2)?] No reaction between wells 1 and 2 Partial identity between wells 1 and 2 Nonidentity between wells 1 and 2 Identity between wells 1 and 2 None . [You are about to perform routine venipuncture on a patient with no known allergy to antiseptics. Which of the following substances would you use to clean the site?] 5.25% sodium hypochlorite 70% isopropyl alcohol Antibacterial soap and water Povidone–iodine None . [Organisms that should be considered in a waterborne outbreak of diarrheal disease include:] Giardia lamblia and Cryptosporidium spp. Endolimax nana and Entamoeba histolytica Blastocystis hominis and Trichomonas vaginalis Toxoplasma gondii and Schistosoma mansoni None . Time's up
