Welcome to your MLT- Professional Name: Email: Phone Number: [Which of the following laboratory professionals is specified by CLIA’88 as responsible for the administration of a clinical area?] Laboratory manager Medical laboratory scientist Medical laboratory technician Technical supervisor None . [Which isoenzyme of ALP migrates farthest toward the anode when electrophoresed at pH 8.6?] Placental Bone Liver Intestinal None . [In a patient with diarrhea, occasionally Entamoeba histolytica/E. dispar (four nucleated cysts, no chromatoidal bars) are identified as being present; however, these cells, which are misdiagnosed as protozoa, are really:] Macrophages Polymorphonuclear leukocytes Epithelial cells Eosinophils None . [Which of the following hemoglobins migrates to the same position as Hgb A2 at pH 8.6?] Hgb H Hgb F Hgb C Hgb S None . [An immunocompromised patient continues to have diarrhea after repeated ova and parasites (O&P) examinations (sedimentation concentration, trichrome permanent stained smear) were reported as negative; organisms that might be responsible for the diarrhea include:] Cryptosporidium spp., Giardia lamblia, and Cystoisospora belli Giardia lamblia, microsporidia, and Endolimax nana Taenia solium and Endolimax nana Cryptosporidium spp. and microsporidia None . [Which group of molds can be ruled out when septate hyphae are observed in a culture?] Dematiaceous Zygomycetes Dermatophytes Dimorphic molds None . [Which of the following tests requires an arterialized specimen?] Bilirubin CBGs Electrolytes Glucose None . [Which technique is used to detect DNA containing a specific base sequence by applying a labeled probe to DNA bands immobilized onto nitrocellulose paper following electrophoresis?] Southern blot Northern blot Dot blot Western blot None . [SITUATION: A 22S QC error occurs for serum calcium by atomic absorption. Fresh standards prepared in 5.0% w/v albumin are found to be linear, but repeating the controls with fresh material does not improve the QC results. Select the most likely cause of this problem.] Matrix effect caused by a viscosity difference between the standards and QC sera Chemical interference caused incomplete atomization Incomplete deconjugation of protein-bound calcium Ionization interference caused by excessive heat None . [What is the most likely explanation when antibody tests for HIV are negative but a polymerase chain reaction test performed 1 week later is positive?] Probably not HIV infection Patient is in the “window phase” before antibody production Tests were performed incorrectly Clinical signs may be misinterpreted None . [The specialty that treats disorders of old age is called:] cardiology. gerontology. pathology. psychiatry. None . [Which of the following is least likely to cause a false-positive result with turbidimetric protein tests?] Tolbutamide X-ray contrast media Penicillin or sulfa antibiotics Ascorbic acid None . [Why might it be necessary for both pink (lactose-positive) and colorless (lactose-negative) colonies from an initial stool culture on MacConkey agar to be subcultured and tested further for possible pathogens?] Most Shigella strains are lactose positive Most Salmonella strains are maltose negative Most Proteus spp. are lactose negative Pathogenic E. coli can be lactose positive or lactose negative None . [The JAK2(V617F) mutation may be positive in all of the following chronic myeloproliferative disorders except:] Essential thrombocythemia Idiopathic myelofibrosis PV CML None . [Congenital dyserythropoietic anemias (CDAs) are characterized by:] Bizarre multinucleated erythroblasts Cytogenetic disorders Megaloblastic erythropoiesis An elevated M:E ratio None . [Early equipment used for bloodletting included all of the following except the:] fleam. hemostat. lancet. leech. None . [The “e” is pronounced separately in:] diastole. syncope. systole. all of the above. None . [Which of the following platelet aggregating agents demonstrates a monophasic aggregation curve when used in optimal concentration?] Thrombin Collagen Adenosine diphosphate (ADP) Epinephrine None . [SITUATION: A 6-year-old child being treated with phenytoin was recently placed on valproic acid for better control of seizures. After displaying signs of phenytoin toxicity including ataxia, a stat phenytoin is determined to be 15.0 mg/L (reference range 10–20 mg/L). A peak blood level drawn 5 hours after the last dose is 18.0 mg/L. The valproic acid measured at the same time is within therapeutic limits. Quality control is within acceptable limits for all tests, but the physician questions the accuracy of the results. What is the most appropriate next course of action?] Repeat the valproic acid level using the last specimen Repeat the phenytoin on both trough and peak samples using a different method Recommend measurement of free phenytoin using the last specimen Recommend a second trough level be measured None . [Which of the following would be omitted from a lab-generated computer label?] Collector’s initials Patient diagnosis Patient identifi cation Time of collection None . [A germ tube–negative, pink yeast isolate was recovered from the respiratory secretions and urine of a patient with AIDS. Given the following results, what is the most likely identification? CORNMEAL TWEEN 80 AGAR Blastoconidia = + Pseudohyphae = Neg Arthroconidia= Neg Urease = +] Candida albicans Rhodotorula spp. Cryptococcus spp. Trichosporon spp. None . [Which of the following enzymes can be used to measure plasma or serum salicylate?] Peroxidase Salicylate esterase Salicylate hydroxylase p-Aminosalicylate oxidase None . [What course of action should be taken if a medical laboratory scientist inadvertently irradiates a unit of red cells twice?] Issue the unit Discard the unit Change the expiration date; then issue the unit Note on the irradiation sticker that the unit was irradiated twice and issue None . [What antibodies are represented by the nucleolar pattern in the immunofluorescence test for antinuclear antibodies?] Antihistone antibodies Anti-dsDNA antibodies Anti-ENA (anti-Sm and anti-RNP) antibodies Anti-RNA antibodies None . [Which of the following is the most effective serial sampling time for ruling out AMI using both myoglobin and a cardiac specific marker in an emergency department environment?] Admission and every hour for the next 3 hours or until positive Admission, 2 hours, 4 hours, and 6 hours or until positive Admission, 3 hours, 6 hours, and a final sample within 12 hours Admission and one sample every 8 hours for 48 hours None . [In essential thrombocythemia, the platelets are:] Increased in number and functionally abnormal Normal in number and functionally abnormal Decreased in number and functional Decreased in number and functionally abnormal None . [The following results were obtained on a 51-year-old male with hepatitis C: Anti-A Anti-B Anti-D A1 cells B cells 4+ 4+ 3+ 0 0 What should be done next?] Retype the patient’s sample to confirm group AB positive Repeat the Rh typing Run a saline control in forward grouping Report the patient as group AB, Rh positive None . [What is the correct procedure upon receipt of a test request for human chorionic gonadotropin (hCG) on the serum from a 60-year-old man?] Return the request; hCG is not performed on men Perform a qualitative hCG test to see if hCG is present Perform the test; hCG may be increased in testicular tumors Perform the test but use different standards and controls None . [Nonpathogenic Moraxella spp. capable of growing on selective media for Neisseria can be differentiated from Neisseria spp. by which test?] Catalase test 10-unit penicillin disk Oxidase test Superoxol test None . [In addition to morphology, cytochemistry, and immunophenotyping, the WHO classification of myelo- and lymphoproliferative disorders is based upon which characteristic?] Proteomics Cytogenetic abnormalities Carbohydrate-associated tumor antigen production Cell signaling and adhesion markers None . Time's up
