New Quiz 10 Dec Welcome to your MLT- Professional Name: Email: Phone Number: [Which of the following proteins has the highest pI?] Albumin Transferrin Ceruloplasmin IgG None . [Nutritionally variant streptococci (NVS) require specific thiol compounds, cysteine, or the active form of vitamin B6. Which of the following tests supplies these requirements?] CAMP test Bacitracin susceptibility test Bile solubility test Staphylococcal cross-streak test None . [Which statement best describes immunofixation electrophoresis (IEF)?] Proteins are separated by electrophoresis followed by overlay of monospecific anti-immunoglobulins Proteins react with monospecific antisera followed by electrophoresis Antisera are electrophoresed, then diffused against patient’s serum Serum is electrophoresed; the separated immunoglobulins diffuse against specific antisera placed into troughs None . [Plate 18 is a peripheral blood film stained with Giemsa’s stain, 1,000×. What condition is suspected from this field?] Macrocytic anemia Agranulocytosis Relapsing fever Lead poisoning None Comment . [The plural form of atrium is:] atri. atria. atrial. atrices. None . [Patient HistoryA 12-year-old white male has the following symptoms: visible bruising on arms and legs, bruising after sports activities, and excessive postoperative hemorrhage following tonsillectomy 3 months ago. His family history revealed that his mother suffers from heavy menstrual bleeding, and his maternal grandfather had recurrent nosebleeds and bruising.These clinical manifestations and laboratory results are consistent with which diagnosis?] Factor VIII deficiency von Willebrand’s disease Glanzmann’s thrombasthenia Bernard–Soulier syndrome None Comment . [The majority of clinical laboratories with a microbiology department should have the capability of serotyping which pathogenic Enterobacteriaceae?] Yersinia enterocolitica, Shigella spp. E. coli O157:H7, Salmonella spp., Shigella spp. Yersinia pestis, Salmonella spp. Edwardsiella spp., Salmonella spp. None . [What term describes the change in shape of erythrocytes seen on a Wright’s-stained peripheral blood smear?] Poikilocytosis Anisocytosis Hypochromia Polychromasia None . [A 19-year-old girl came to her physician complaining of a sore throat and fatigue. Upon physical examination, lymphadenopathy was noted. Reactive lymphocytes were noted on the differential, but a rapid test for IM antibodies was negative. Liver enzymes were only slightly elevated. What test(s) should be ordered next?] Hepatitis testing EBV serological panel HIV confirmatory testing Bone marrow biopsy None . [A PCR analysis of a vaginal sample for Chlamydia trachomatis gives a negative result (optical density of biotinylated reaction product below the cutoff point). The internal control result is also below the cutoff. Positive and negative controls produced acceptable results. What action should be taken?] The test should be reported as negative The sample should be diluted and the test repeated The result should not be reported and the sample should be repeated A preliminary result of negative should be reported but should be confirmed by further testing using a different method of analysis None . [Which of the following enzymes is considered most tissue specific?] Creatine kinase (CK) Amylase Alkaline phosphatase (ALP) Alcohol dehydrogenase (ADH) None . [Which of the following conditions is cause for rejecting an analytical run?] Two consecutive controls greater than 2 s above or below the mean Three consecutive controls greater than 1 s above the mean Four controls steadily increasing in value but less than ±1 s from the mean One control above +1 s and the other below -1 s from the mean None . [What is the PCO2 if the dCO2 is 1.8 mmol/L?] 24 mm Hg 35 mm Hg 60 mm Hg 72 mm Hg None . [The WHO classification requires what percentage for the blast count in the blood or bone marrow for the diagnosis of AML?] At least 30% At least 20% At least 10% Any percentage None . [Why is a chemiluminescent immunoassay (CIA) or enzyme immunoassay (EIA) the method of choice for detection of certain analytes, such as hormones, normally found in low concentrations?] Because of low cross reactivity Because of high specificity Because of high sensitivity Because test systems may be designed as both competitive and noncompetitive assays None . [The tourniquet should be released:] as soon as blood fl ow is established. before needle removal from the arm. within 1 minute of its application. all of the above. None . [Cystitis means:] blueness of the skin. cellular infection. infl ammation of the bladder. pertaining to a cell. None . [Two blood cultures on a newborn grew β-hemolytic streptococci with the following reactions: CAMP test = + Hippurate hydrolysis = + Bile solubility = Neg 6.5% salt = + Bacitracin = Resistant Bile esculin = Neg PYR = Neg Trimethoprim– sulfamethoxazole = Resistant Which is the most likely identification?] Group A streptococci Group B streptococci Group D streptococci Nongroup A, nongroup B, nongroup D streptococci None . [Proximal is defi ned as:] away from the middle. closest to the middle. farthest from the center. nearest to the point of attachment. None . [SITUATION: An amylase result is 550 U/L. A 1:4 dilution of the specimen in NaCl gives 180 U/L (before mathematical correction for dilution). The dilution is repeated with the same results. The technologist should:] Report the amylase as 550 U/L Report the amylase as 720 U/L Report the amylase as 900 U/L Dilute the sample 1:10 in distilled water and repeat None . [The macrocytes typically seen in megaloblastic processes are:] Crescent-shaped Teardrop-shaped Ovalocytic Pencil-shaped None . [Given the following urinalysis results, select the most appropriate course of action: pH = 8.0 Protein = Trace Glucose = Neg Ketone = Small Blood = Neg Nitrite = Neg Microscopic findings: RBCs = 0–2/HPF WBCs = 20–50/HPF Bacteria = Large Crystals = Small, CaCO3] Call for a new specimen because urine was contaminated in vitro Recheck pH because calcium carbonate (CaCO3) does not occur at alkaline pH No indication of error is present; results indicate a UTI Report all results except bacteria because the nitrite test was negative None . [In which age group would 60% lymphocytes be a normal finding?] 6 months–2 years 4–6 years 11–15 years 40–60 years None . [Plate 15 is an iodine-stained fecal specimen seen under 400× using brightfield microscopy. The plate shows the ovum of which parasite?] Pinworm Threadworm Hookworm Whipworm None Comment . [In the test for urease production, ammonia reacts to form which product?] Ammonium citrate Ammonium carbonate Ammonium oxalate Ammonium nitrate None . [Hyperalbuminemia is caused by:] Dehydration syndromes Liver disease Burns Gastroenteropathy None . [Persistent noise from an ion-selective electrode is most often caused by:] Contamination of sample Blocked junction at the salt bridge Overrange from high concentration Improper calibration None . [Examination of 24-hour unpreserved urine specimen is sometimes helpful in the recovery of:] Trichomonas vaginalis trophozoites Schistosoma haematobium eggs Enterobius vermicularis eggs Strongyloides stercoralis larvae None . [Which of the following specimens would be acceptable for testing?] CBC collected in a lavender-top tube Potassium specimen that is hemolyzed Protime specimen in a partially filled tube Specimen lacking an identification label None . [A manual RBC count is performed on a pleural fluid. The RBC count in the large center square of the Neubauer hemacytometer is 125, and the dilution is 1:200. What is the total RBC count?] 27.8 × 10^9/L 62.5 × 10^9/L 125.0 × 10^9/L 250.0 × 10^9/L None Comment . Time's up